T5168
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 | Immunofluorescence Human DAOY cells were stained using Monoclonal Anti-α-Tubulin, clone no. B-5-1-2 (Cat. No. T5168) with Anti-Mouse-FITC (green) and Hoechst 33528 (blue). From Yuying Jiang, Columbus Children’s Research Institute, Columbus, OH. |
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T5168
Sigma
Monoclonal Anti-α-Tubulin antibody produced in mouse
ascites fluid, clone B-5-1-2
| MDL number: | MFCD00145891 |
Description
| Immunogen | Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes. |
| Specificity | Recognizes an epitope located at the C-terminal end of the α-tubulin isoform in a variety of organisms. |
Properties
| antibody form | ascites fluid |
| clone | B-5-1-2 (monoclonal) |
| mol wt | antigen mol wt ~50 kDa |
| contains | 15 mM sodium azide |
| species reactivity | human, Chlamydomonas, African green monkey, chicken, mouse, bovine, rat, kangaroo rat, sea urchin |
| application(s) | indirect immunofluorescence: 1:2,000 using cultured human or chicken fibroblasts |
| radioimmunoassay: suitable | |
| isotype | IgG1 |
| Gene Information | human ... TUBA4A(7277) |
| rat ... Tuba1a(64158) | |
| mouse ... Tuba1a(22142) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
Related Products
| Array | CSAA1, Panorama® Antibody Microarray - Cell Signaling Kit |
References
| reference | LeDizet, M., and Piperno, G., Detection of acetylated α-tubulin by specific antibodies. Meth. Enzymol. 196, 264, (1991) Abstract |
| Yuen, C.Y., et al., Loss-of-Function Mutations of Root Hair Defective3 Suppress Root Waving, Skewing, and Epidermal Cell File Rotation in Arabidopsis1 Plant Physiol. 138, 701-714, (2005) Abstract | |
| Bouchard, C., et al., FoxO transcription factors suppress Myc-driven lymphomagenesis via direct activation of Arf Genes Dev. 21, 2775-2787, (2007) Abstract | |
| Ralph, E., et al., DNA damage induces Cdt1 proteolysis in fission yeast through a pathway dependent on Cdt2 and Ddb1 EMBO Rep. 7, 1134-1139, (2006) Abstract |
